H+ATPase antibody, rabbit polyclonal

Background

The Plasma Membrane H+ATPase is a family of proteins of ca. 100 kDa that are believed to be exclusive to the

plasma membranes of plants and fungi. The protein is anchored within biological membrane which creates an

electrochemical gradient used as an energy source and is essential for uptake of most metabolites and plant

responses to environment, for example movement of leaves. Cellular compartment marker for plasma membrane.

Other names:

AHA3, ARABIDOPSIS THALIANA ARABIDOPSIS H(+)-ATPASE, ATAHA3, H(+)-ATPASE 3, HA3

Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana H+ATPase proteins

(At5g57350, AHA3).

Concentration: 1.0mg/ml

Tested applications: Western blot (WB), IF

Expected/apparent MW: 95 kDa (Arabidopsis thaliana)

Species specificity: Arabidopsis thaliana

Recommended dilution: 1 : 500-2000 (WB, detect endogenous protein ), 1: 100 - 1: 500 (IF)(optimal dilutions

should be determined by the end use.)

Storage Buffer: PBS Buffer, pH7.4

Storage: Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Stable for 2

years from date of receipt. Aliquot to avoid repeated freezing and thawing.

* Please note: FOR RESEARCH USE ONLY