Rbcl（Rubisco large subunit）antibody, rabbit polyclonal

Background

Rubisco catalyzes the rate-limiting step of CO2 fixation in photosynthetic organisms. It is demonstrably

homologous from purple bacteria to flowering plants and consists of two protein subunits, each present in 8 copies.

In plants and green algae, the large subunit (~55 kDa) is coded by the chloroplast rbcL gene, and the small subunit

(15 kDa) is coded by a family of nuclear rbcS genes.

Immunogen: KLH-conjugated synthetic peptide conserved across all known plant, algal and (cyano) bacterial

RbcL protein sequences (ATCG00490).

Concentration: 1.0mg/ml

Expected/apparent MW: 55 kDa

Tested applications: Western blot (WB), IF

Species specificity: Arabidopsis thaliana, Nicotiana tabacum, Oryza sativa, Zea mays

Recommended dilution: 1 : 500-2000 (WB, detect endogenous protein ), 1: 100 - 1: 500 (IF)(optimal dilutions

should be determined by the end use.)

Storage Buffer: PBS Buffer, pH7.4

Storage: Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Stable for 2

years from date of receipt. Aliquot to avoid repeated freezing and thawing